1. 1 light microscopy showshow cells move and behave
  2. 2 electron microscopy showshow complexes arrange in cells
  3. 3 single particle reconstruction showshow proteins arrange in complexes
  4. 4 X-ray crystallography showshow atoms arrange in proteins

Summary

Concept Check Questions

• How can you locate a protein of interest by light microscopy?

• What happens to liquid water in a vacuum?

• What are gold beads used for in cryo-ET?

• Which technique can reveal the atomic structure of a small protein?

Further Reading

• Jensen. Getting Started in Cryo-EM Video Lectures [15].

• Oikonomou and Jensen (2017). Cellular electron cryotomography: toward structural biology in situ [16].

• Ruska (1987). Nobel Lecture: The development of the electron microscope and of electron microscopy [17].

• Tsien (2005). Breeding molecules to spy on cells [18].